Studies on Autolysis of Clostridium botulinum Type A

Serological studies of Clostridium botulinum type E and related organisms.

Clostridium botulinum type E antigens prepared from washed cells by either Formalin treatment or heating at 100 C were used for immunizing rabbits. Agglutination tests showed that high levels of antibody were produced by both types of preparations. Flagellar antigens were highly strain-specific, whereas the somatic antigens were sufficiently similar to produce complete cross-agglutination. One ...

Purification of Clostridium botulinum type A neurotoxin.

production and crystallization of clostridium botulinum type a toxin

introduction: widespread medical usage of botulinum toxins has increased the need for the precise analysis of its biological activity. the aim of this study was production and crystallization of the botulinum type a toxins. methods: in this study, clostridium botulinum was grown and inoculated into the tripticase soya broth culture media, under anaerobic condition and toxin production was monit...

Cloning, Expression and Purification of Clostridium botulinum Neurotoxin Type E Binding Domain

Clostridium botulinum Type D Lysogenized Organisms

We examined production of ADP-ribosyltransferase C3 in 11 strains of Clostridium botldinum type C and D and their nontoxigenic derivatives. Antisera to C3 proteins of type C organisms divided C3 proteins roughly into at least two groups, bearing no relation to their bacterial types. The C3 gene of type D strain South African was isolated from a toxigenic phage library, and the complete sequence...